Jerome Irianto Ph.D.
Assistant Professor
Biosketch
Irianto Lab page
Mechanobiology has been the common theme throughout Dr. Irianto research career. His graduate work looked at the consequence of osmotic challenge on chondrocytes. He discovered that alterations in osmolality leads to changes in chromosome condensation state that dictate nuclear enzymes accessibility and translates to changes in transcription as measured by microarray. For Dr. Irianto's postdoctoral work, still focusing on mechanobiology, he entered the cancer biology field by studying the consequence of constrictions in cancer cell migration. Here, he showed that constricted migration of an osteosarcoma line causes damages to the nucleus and DNA, followed by lasting and inheritable genomic, transcriptomic and phenotypic changes, which may contribute to the inter/intra tumor heterogeneity observed in the disease. In 2018, Dr. Irianto joined Department of Biomedical Sciences, Florida State University College of Medicine.
Education
Postdoctoral Research, Department of Chemical and Biomolecular Engineering, University of Pennsylvania
PhD, Institute of Bioengineering, Queen Mary, University of London
MEng, School of Engineering and Materials Science, Queen Mary, University of London
Honors/Awards
2021 , Trans-network Physical Sciences in Oncology (WFU-FSU), NCI/NIH
2021 , William G. "Bill" Bankhead, Jr., and David Coley Cancer Research Program, FL DOH
2021 , Multidisciplinary Support Grant Program, CRC FSU
2019 , First-Year Assistant Professor Award, CRC FSU
2017 , Trans-network Physical Sciences in Oncology (Penn-Columbia), NCI/NIH
2008 , QMUL College DTA Studentship
2008 , Howard Kidman Prize
2006 , Anthony Gordon Pooley Memorial Prize
Memberships
Biophysical Society
American Society for Cell Biology
Biomedical Engineering Society
American Association for Cancer Research
Research Focus
Cancer mechanobiology and genomics
Publications
Xie, G., Walker III, R. R., & Irianto, J. (2020). Nuclear mechanosensing: mechanism and consequences of a nuclear rupture. Mutation Research. https://doi.org/10.1016/j.mrfmmm.2020.111717.
Bijonowski, B. M., Fu, Q., Yuan, X., Irianto, J., Li, Y., Grant, S. C., & Ma, T. (2020). Aggregation-induced integrated stress response rejuvenates culture-expanded human mesenchymal stem cells. Biotechnology and Bioengineering, 117(10):3136-3149. doi: 10.1002/bit.27474
Xia, Y., Pfeifer, C. R., Zhu, K., Irianto, J., Liu, D., Pannell, K., Chen, E. J., Dooling, L. J., Tobin, M. P., Wang, M., Ivanovska, I. L., Smith, L. R., Greenberg, R. A., & Discher, D. E. (2019). Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle. J Cell Biol, 218(8), 2545-2563.
L.R. Smith, J.Irianto, Y. Xia, C.R. Pfeifer, and D.E. Discher (2019). "Constricted migration modulates stem cell differentiation". Molecular Biology of the Cell. doi.org/10.1091/mbc.E19-02-0090
S. Cho, M. Vashisth, A. Abbas, S. Majkut, K. Vogel, Y. Xia, I.L. Ivanovska, J.Irianto, M. Tewari, K. Zhu, E.D. Tichy, F. Mourkioti, H.Y. Tang, R.A. Greenberg, B.L. Prosser, D.E. Discher (2019). "Mechanosensing by the Lamina Protects against Nuclear Rupture, DNA Damage, and Cell-Cycle Arrest". Developmental Cell. 49(6):920-935.
Y Xia, C.R. Pfeifer, S. Cho, D.E. Discher, J.Irianto (2018). “Nuclear mechanosensing”. Emerging Topics in Life Sciences. 2(5):713-725.
Y. Xia*, I. Ivanovska*, K. Zhu, L. Smith, C. Alvey, J.Irianto, C.R. Pfeifer, J. Ji, D. Liu, S. Cho, R. Bennett, A.J. Liu, R. Greenberg, and D. Discher (2018). “Nuclear rupture at sites of high curvature compromises retention of DNA repair factors”. Journal of Cell Biology. doi: 10.1083/jcb.201711161
C.R. Pfeifer, Y. Xia, K. Zhu, D. Liu, J.Irianto, V.M.M. García, L.M.S. Millán, B. Niese, S.M. Harding, D. Deviri, R.A. Greenberg, and D.E. Discher (2018). “Constricted migration increases DNA damage and independently represses cell cycle”. Molecular Biology of the Cell. doi: doi.org/10.1091/mbc.E18-02-0079
S. Cho, A. Abbas, J.Irianto, I. Ivanovska, M. Tewari, D.E. Discher (2018). “Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells”. Nucleus. 9(1):230-245.
P.R. Nair, C. Alvey, X. Jin, J.Irianto, I. Ivanovska, D.E. Discher (2018). “Filomicelles deliver a chemo-differentiation combination of paclitaxel and retinoic acid that durably represses carcinomas in liver to prolong survival”. Bioconjugate Chemistry. 29(4):914-927
A. Buxboim, J.Irianto, J. Swift, A. Athirasala, J-W. Shin, F. Rehfeldt, D.E. Discher (2017). “Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes”. Molecular Biology of the Cell. 28(23):3333-3348
S.M. Harding, J.L. Benci, J.Irianto, D.E. Discher, A.J. Minn, R.A. Greenberg (2017). “Mitotic progression following DNA damage enables pattern recognition within micronuclei”. Nature. 548:466-470
C. Alvey, K. Spinler, J.Irianto, C.R. Pfeifer, B. Hayes, Y. Xia, S. Cho, P.C.P.D. Dingal, M. Tewari, D.E Discher (2017). “SIRPA-inhibited, marrow-derived macrophages engorge, accumulate, and differentiate in antibody-targeted regression of solid tumors”. Current Biology. 27(14):2065-2077
R.R. Bennett, C.R. Pfeifer, J.Irianto, Y. Xia, D.E. Discher, A.J. Liu (2017). “Elastic-fluid model for DNA damage and mutation from nuclear fluid segregation due to cell migration”. Biophysical Journal. 112(11):2271-2279
C.R. Pfeifer, C.M. Alvey, J.Irianto, D.E. Discher (2017). “Genome variation across cancers scales with tissue stiffness – an invasion-mutation mechanism and implications for immune cell infiltration”. Current Opinion in Systems Biology. 2:102-113
S. Cho, J.Irianto, D.E. Discher (2017). “Mechanosensing by the nucleus: From pathways to scaling relationships”. Journal of Cell Biology. doi: https://doi.org/10.1083/jcb.201610042
J.Irianto, Y. Xia, C.R. Pfeifer, A. Athirasala, J. Ji, C.M. Alvey, M. Tewari, R.R. Bennett, S.M. Harding, A.J. Liu, R.A. Greenberg, D.E. Discher (2017). “DNA damage follows repair factor depletion and portends genome variation in cancer cells after pore migration”. Current Biology. 27(2):210-223
J.Irianto, Y. Xia, C.R. Pfeifer, R.A. Greenberg, D.E. Discher (2017). “As a nucleus enters a small pore, chromatin stretches and maintains integrity even with DNA breaks”. Biophysical Journal. 112(3): 446-449
J.Irianto*, C.R. Pfeifer*, R.R. Bennett, Y. Xia, I.L. Ivanovska, A.J. Liu, R.A. Greenberg, D.E. Discher (2016). “Segregation of mobile nuclear proteins away from chromatin when the nucleus is constricted”. Molecular Biology of the Cell. 27(25):4011-4020
J.Irianto, C.R. Pfeifer, Y. Xia, D.E. Discher (2016). “SnapShot: Mechanosensing Matrix”. Cell. 165(7):1820
J.Irianto, C.R. Pfeifer, I.L. Ivanovska, J. Swift, D.E. Discher (2016). “Nuclear lamins in cancer”. Cellular and Molecular Bioengineering. 9(2):258-267
J.Irianto, I.L. Ivanovska, J. Swift, D.E. Discher (2016). “The nuclear lamina: from mechanosensing in differentiation to cancer cell migration”. Molecular and Cellular Mechanobiology. p175-195
Z. Wang, J.Irianto, S. Kazun, W. Wang, M.M. Knight (2014). "The rate of hypo-osmotic challenge influences regulatory volume decrease (RVD) and mechanical properties of articular chondrocytes". Osteoarthritis and Cartilage. 23(2): 289-299
A. Buxboim*, J. Swift*, J.Irianto, K. Spinler, P.C.D.P. Dingal, A. Athirasala, Y-R.C. Kao, S. Cho, T. Harada, J-W. Shin, D.E. Discher (2014). "Matrix elasticity regulates lamin-A, C phosphorylation and turnover with feedback to actomyosin". Current Biology. 24(16):1909-1917
I. Ivanovska, J. Swift, J.Irianto, K. Spinler, J-W. Shin, A. Buxboim, D.E. Discher (2014). "Nuclear Imaging in Mechanobiology". Handbook of Imaging in Biological Mechanics. p397-409.
T. Harada, J. Swift, J.Irianto, J-W. Shin, K.R. Spinler, A. Athirasala, R. Diegmiller, P.C.D.P. Dingal, I.L. Ivanovska, D.E. Discher (2014). "Nuclear lamin stiffness is a barrier to 3D migration, but softness can limit survival". Journal of Cell Biology. 204(5):669-682
J.Irianto, D.A. Lee, M.M. Knight (2014). “Quantification of Chromatin Condensation Level by Image Processing”. Medical Engineering & Physics. 36(3): 412-417
J.Irianto, G. Ramaswamy, R. Serra, M.M. Knight (2014). "Depletion of primary cilia reduces the compressive modulus of articular cartilage". Journal of Biomechanics. 47(2): 579-582
J.Irianto, J. Swift, R.P. Martins, G.D. McPhail, M.M. Knight, D.E. Discher, D.A. Lee (2013). “Osmotic Challenge Drives Rapid and Reversible Chromatin Condensation in Chondrocytes”. Biophysical Journal. 104(4): 759-769.
J. Chen, J.Irianto, S. Inamdar, P. Pravincumar, D.A. Lee, D.L. Bader, M.M. Knight (2012). “Cell mechanics, structure, and function are regulated by the stiffness of the three-dimensional microenvironment”. Biophysical Journal. 103(6): 1188-97.
Irianto Lab page
Mechanobiology has been the common theme throughout Dr. Irianto research career. His graduate work looked at the consequence of osmotic challenge on chondrocytes. He discovered that alterations in osmolality leads to changes in chromosome condensation state that dictate nuclear enzymes accessibility and translates to changes in transcription as measured by microarray. For Dr. Irianto's postdoctoral work, still focusing on mechanobiology, he entered the cancer biology field by studying the consequence of constrictions in cancer cell migration. Here, he showed that constricted migration of an osteosarcoma line causes damages to the nucleus and DNA, followed by lasting and inheritable genomic, transcriptomic and phenotypic changes, which may contribute to the inter/intra tumor heterogeneity observed in the disease. In 2018, Dr. Irianto joined Department of Biomedical Sciences, Florida State University College of Medicine.
Postdoctoral Research, Department of Chemical and Biomolecular Engineering, University of Pennsylvania
PhD, Institute of Bioengineering, Queen Mary, University of London
MEng, School of Engineering and Materials Science, Queen Mary, University of London
2021 , Trans-network Physical Sciences in Oncology (WFU-FSU), NCI/NIH
2021 , William G. "Bill" Bankhead, Jr., and David Coley Cancer Research Program, FL DOH
2021 , Multidisciplinary Support Grant Program, CRC FSU
2019 , First-Year Assistant Professor Award, CRC FSU
2017 , Trans-network Physical Sciences in Oncology (Penn-Columbia), NCI/NIH
2008 , QMUL College DTA Studentship
2008 , Howard Kidman Prize
2006 , Anthony Gordon Pooley Memorial Prize
Biophysical Society
American Society for Cell Biology
Biomedical Engineering Society
American Association for Cancer Research
Cancer mechanobiology and genomics
Xie, G., Walker III, R. R., & Irianto, J. (2020). Nuclear mechanosensing: mechanism and consequences of a nuclear rupture. Mutation Research. https://doi.org/10.1016/j.mrfmmm.2020.111717.
Bijonowski, B. M., Fu, Q., Yuan, X., Irianto, J., Li, Y., Grant, S. C., & Ma, T. (2020). Aggregation-induced integrated stress response rejuvenates culture-expanded human mesenchymal stem cells. Biotechnology and Bioengineering, 117(10):3136-3149. doi: 10.1002/bit.27474
Xia, Y., Pfeifer, C. R., Zhu, K., Irianto, J., Liu, D., Pannell, K., Chen, E. J., Dooling, L. J., Tobin, M. P., Wang, M., Ivanovska, I. L., Smith, L. R., Greenberg, R. A., & Discher, D. E. (2019). Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle. J Cell Biol, 218(8), 2545-2563.
L.R. Smith, J.Irianto, Y. Xia, C.R. Pfeifer, and D.E. Discher (2019). "Constricted migration modulates stem cell differentiation". Molecular Biology of the Cell. doi.org/10.1091/mbc.E19-02-0090
S. Cho, M. Vashisth, A. Abbas, S. Majkut, K. Vogel, Y. Xia, I.L. Ivanovska, J.Irianto, M. Tewari, K. Zhu, E.D. Tichy, F. Mourkioti, H.Y. Tang, R.A. Greenberg, B.L. Prosser, D.E. Discher (2019). "Mechanosensing by the Lamina Protects against Nuclear Rupture, DNA Damage, and Cell-Cycle Arrest". Developmental Cell. 49(6):920-935.
Y Xia, C.R. Pfeifer, S. Cho, D.E. Discher, J.Irianto (2018). “Nuclear mechanosensing”. Emerging Topics in Life Sciences. 2(5):713-725.
Y. Xia*, I. Ivanovska*, K. Zhu, L. Smith, C. Alvey, J.Irianto, C.R. Pfeifer, J. Ji, D. Liu, S. Cho, R. Bennett, A.J. Liu, R. Greenberg, and D. Discher (2018). “Nuclear rupture at sites of high curvature compromises retention of DNA repair factors”. Journal of Cell Biology. doi: 10.1083/jcb.201711161
C.R. Pfeifer, Y. Xia, K. Zhu, D. Liu, J.Irianto, V.M.M. García, L.M.S. Millán, B. Niese, S.M. Harding, D. Deviri, R.A. Greenberg, and D.E. Discher (2018). “Constricted migration increases DNA damage and independently represses cell cycle”. Molecular Biology of the Cell. doi: doi.org/10.1091/mbc.E18-02-0079
S. Cho, A. Abbas, J.Irianto, I. Ivanovska, M. Tewari, D.E. Discher (2018). “Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells”. Nucleus. 9(1):230-245.
P.R. Nair, C. Alvey, X. Jin, J.Irianto, I. Ivanovska, D.E. Discher (2018). “Filomicelles deliver a chemo-differentiation combination of paclitaxel and retinoic acid that durably represses carcinomas in liver to prolong survival”. Bioconjugate Chemistry. 29(4):914-927
A. Buxboim, J.Irianto, J. Swift, A. Athirasala, J-W. Shin, F. Rehfeldt, D.E. Discher (2017). “Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes”. Molecular Biology of the Cell. 28(23):3333-3348
S.M. Harding, J.L. Benci, J.Irianto, D.E. Discher, A.J. Minn, R.A. Greenberg (2017). “Mitotic progression following DNA damage enables pattern recognition within micronuclei”. Nature. 548:466-470
C. Alvey, K. Spinler, J.Irianto, C.R. Pfeifer, B. Hayes, Y. Xia, S. Cho, P.C.P.D. Dingal, M. Tewari, D.E Discher (2017). “SIRPA-inhibited, marrow-derived macrophages engorge, accumulate, and differentiate in antibody-targeted regression of solid tumors”. Current Biology. 27(14):2065-2077
R.R. Bennett, C.R. Pfeifer, J.Irianto, Y. Xia, D.E. Discher, A.J. Liu (2017). “Elastic-fluid model for DNA damage and mutation from nuclear fluid segregation due to cell migration”. Biophysical Journal. 112(11):2271-2279
C.R. Pfeifer, C.M. Alvey, J.Irianto, D.E. Discher (2017). “Genome variation across cancers scales with tissue stiffness – an invasion-mutation mechanism and implications for immune cell infiltration”. Current Opinion in Systems Biology. 2:102-113
S. Cho, J.Irianto, D.E. Discher (2017). “Mechanosensing by the nucleus: From pathways to scaling relationships”. Journal of Cell Biology. doi: https://doi.org/10.1083/jcb.201610042
J.Irianto, Y. Xia, C.R. Pfeifer, A. Athirasala, J. Ji, C.M. Alvey, M. Tewari, R.R. Bennett, S.M. Harding, A.J. Liu, R.A. Greenberg, D.E. Discher (2017). “DNA damage follows repair factor depletion and portends genome variation in cancer cells after pore migration”. Current Biology. 27(2):210-223
J.Irianto, Y. Xia, C.R. Pfeifer, R.A. Greenberg, D.E. Discher (2017). “As a nucleus enters a small pore, chromatin stretches and maintains integrity even with DNA breaks”. Biophysical Journal. 112(3): 446-449
J.Irianto*, C.R. Pfeifer*, R.R. Bennett, Y. Xia, I.L. Ivanovska, A.J. Liu, R.A. Greenberg, D.E. Discher (2016). “Segregation of mobile nuclear proteins away from chromatin when the nucleus is constricted”. Molecular Biology of the Cell. 27(25):4011-4020
J.Irianto, C.R. Pfeifer, Y. Xia, D.E. Discher (2016). “SnapShot: Mechanosensing Matrix”. Cell. 165(7):1820
J.Irianto, C.R. Pfeifer, I.L. Ivanovska, J. Swift, D.E. Discher (2016). “Nuclear lamins in cancer”. Cellular and Molecular Bioengineering. 9(2):258-267
J.Irianto, I.L. Ivanovska, J. Swift, D.E. Discher (2016). “The nuclear lamina: from mechanosensing in differentiation to cancer cell migration”. Molecular and Cellular Mechanobiology. p175-195
Z. Wang, J.Irianto, S. Kazun, W. Wang, M.M. Knight (2014). "The rate of hypo-osmotic challenge influences regulatory volume decrease (RVD) and mechanical properties of articular chondrocytes". Osteoarthritis and Cartilage. 23(2): 289-299
A. Buxboim*, J. Swift*, J.Irianto, K. Spinler, P.C.D.P. Dingal, A. Athirasala, Y-R.C. Kao, S. Cho, T. Harada, J-W. Shin, D.E. Discher (2014). "Matrix elasticity regulates lamin-A, C phosphorylation and turnover with feedback to actomyosin". Current Biology. 24(16):1909-1917
I. Ivanovska, J. Swift, J.Irianto, K. Spinler, J-W. Shin, A. Buxboim, D.E. Discher (2014). "Nuclear Imaging in Mechanobiology". Handbook of Imaging in Biological Mechanics. p397-409.
T. Harada, J. Swift, J.Irianto, J-W. Shin, K.R. Spinler, A. Athirasala, R. Diegmiller, P.C.D.P. Dingal, I.L. Ivanovska, D.E. Discher (2014). "Nuclear lamin stiffness is a barrier to 3D migration, but softness can limit survival". Journal of Cell Biology. 204(5):669-682
J.Irianto, D.A. Lee, M.M. Knight (2014). “Quantification of Chromatin Condensation Level by Image Processing”. Medical Engineering & Physics. 36(3): 412-417
J.Irianto, G. Ramaswamy, R. Serra, M.M. Knight (2014). "Depletion of primary cilia reduces the compressive modulus of articular cartilage". Journal of Biomechanics. 47(2): 579-582
J.Irianto, J. Swift, R.P. Martins, G.D. McPhail, M.M. Knight, D.E. Discher, D.A. Lee (2013). “Osmotic Challenge Drives Rapid and Reversible Chromatin Condensation in Chondrocytes”. Biophysical Journal. 104(4): 759-769.
J. Chen, J.Irianto, S. Inamdar, P. Pravincumar, D.A. Lee, D.L. Bader, M.M. Knight (2012). “Cell mechanics, structure, and function are regulated by the stiffness of the three-dimensional microenvironment”. Biophysical Journal. 103(6): 1188-97.